Journal: Integrative biology : quantitative biosciences from nano to macro
Article Title: Bioreactor Model of Neuromuscular Junction with Electrical Stimulation for Pharmacological Potency Testing
doi: 10.1039/c7ib00144d
Figure Lengend Snippet: GFP+, CD2+ motoneurons derived from mouse ES cell line (HBG3) and myotubes transdifferentiated from MEFs were cocultured in a culture chamber integrated with electrical stimulation with the timeline shown above.
Article Snippet: Magnetic-activated cell sorting Upon dissociation and wash with L15 medium consisting of L15 solution (Sigma), 50 μM EDTA, 4% BSA (Sigma), 25 mM glucose, 2% Horse Serum (Sigma), 40 μg/mL DNase, 500 μg/ML Insulin (Sigma), 0.01M Putrecine (Sigma), 10 mg/mL Conalbumin (Sigma) and 30 μM Sodium Selenite (Sigma), 15 × 10 6 dissociated cells were incubated for 20 minutes at 4 °C in 80 μL of L15 medium supplemented with 10 μL of anti-rat CD2 antibody (Invitrogen).
Techniques: Derivative Assay